Fig. 6.

Synaptophysin (SYN) is decreased in the PVN following RH. A: representative Western blot of SYN expression in the corresponding brain areas of interest (as identified in Fig. 1) from saline and RH-treated animals (n = 4/group). Top: blot probed with α-SYN antibody; bottom: blot probed with α-actin antibody as a control. Separator lines indicate where the image was cropped to include only the brain areas of interest. B: quantitation of Western blot SYN protein expression. *A statistically significant (P < 0.017) decrease in SYN expression is observed in the PVN following RH. Protein levels are corrected to actin expression. ARC, arcuate nucleus of the hypothalamus; D/V, combined DMH/VMH.