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Effect of chronic hypoxia on phosphorylation (p) of myosin phosphatase targeting subunit 1 (MYPT1) at Thr696 (T696). Top: Western blots. Bottom: densitometric scanning of phosphorylated MYPT1 at Thr696 normalized to nonphosphorylated MYPT1. ET-1, endothelin-1, 3 × 10−9 M; 8-Br-cGMP, 10−4 M; PKG-I, Rp-8-Br-PET-cGMPS, 3 × 10−5 M; Y-27632, 10−5 M. Data are shown as means ± SE; n = 8 for each group. *Significantly different from normoxia; †significantly different from basal (P < 0.05).
