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Effect of washout of lubiprostone. X-axis units in hours:minutes elapsed. Lubiprostone (10 μM) (solution 5) supplied to a CFKO mouse produces the characteristic hyperpolarization (n = 2). However, substitution with zero Cl− (solution 3) containing 1% DMSO (vehicle control) results in a loss of effect or washout. This can be repeated several times in the same mouse.
