Table 3.

Inhibition of CFTR does not abolish the chloride transport activated by lubiprostone or ATP

Genotype (n)	Weight, g	Baseline, mV	Amiloride, mV	Zero Cl−, mV +50 μM CFTRinh 172*	Δ Lubi 20 μM, mV	Δ 100 μM ATP, mV
C57Bl/6 (7)	24.7±0.5	−4.0±0.71	−1.6±0.51	−0.7±0.84	−6.5±1.8	−10.0±3.7
				P = 0.0001	P = 0.003
					P = 0.007†

C57Bl/6 mice (n = 7) underwent NPD testing with zero Cl⁻ solution containing CFTRinh 172 before lubiprostone stimulation.

^*Application of CFTR inhibitor before the zero Cl⁻ response resulted in near isoelectric PD. The value Δ Lubi 20 μM reflects the difference of the maximal change induced by lubiprostone and the stable zero Cl⁻ value. The Δ 100 μM ATP value is the difference of maximal ATP response and the stable zero Cl⁻ value, which represents the calcium-activated chloride conductance. Significance was tested by paired t-test for each response compared with zero Cl⁻ containing CFTRinh 172.

^†Paired t-test result for lubiprostone and ATP.