Fig. 2.

Activation of capacitative Ca²⁺ entry (CCE) in fetal and adult PASMCs. Representative traces of [Ca²⁺]_i of myocytes from fetal (A) and adult (B) sheep show effect of extracellular Ca²⁺ removal, addition of 10 μM cyclopiazonic acid (CPA), and sequential exposure to 10 mM caffeine (Caf) and readdition of extracellular Ca²⁺ on cytosolic Ca²⁺. Dashed horizontal line shows basal [Ca²⁺]_i. C: average change in [Ca²⁺]_i from basal levels during readdition of Ca²⁺ in cells from fetal and adult sheep. Values are means ± SE. No significant difference was observed (P = 0.4 by unpaired t-test).