Fig. 7.

Lung tissue heat shock protein 90 (HSP90) expression in sham-operated control and shunted lambs at 2, 4, and 8 wk of age. Top: representative Western blots are shown for HSP90 protein extracts prepared from lung tissue separated on a 7.5% SDS-polyacrylamide gel, electrophoretically transferred to Hybond membranes, and analyzed using a specific antiserum raised against HSP90 and reprobed with β-actin to demonstrate equal loading. Bottom: densitometric values for HSP90 protein shown relative to control. n = 5 for each group. Values are means ± SD.