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Cytokine analysis by Bioplex. Lung samples were prepared by homogenizing lungs in PBS buffer containing a protease inhibitor cocktail (Complete) and 0.05% Triton X-100 nonionic detergent. The debris-free supernatant was prepared using high-speed centrifugation. Samples were assayed using the multiplex proteomics analysis as prescribed by the Bio-Rad protocols. Data represent means ± SE from 8–10 mice/group.
