Fig. 2.
Effect of BMPR2R899X transgene on bone morphogenetic protein type II receptor (BMPR2) signaling pathways. A: A7r5 vascular smooth muscle cells transfected with pBRE luciferase reporter and either wild-type BMPR2 or BMPR2 with R899X mutation with increasing doses of recombinant BMP4. Two-way ANOVA shows a highly significant effect of added ligand (P < 0.0001) but no effect of vector or ligand by vector. B: SMAD1 phosphorylation (pSmad1) is not decreased (P = not significant by unpaired t-test). C: with the same samples and loading order as B, SMAD target Id1 protein remains unchanged. D: p38 phosphorylation increases in whole lung from mice expressing BMPR2R899X as normalized to total p38 (P < 0.05 by unpaired t-test). bAct, β-actin; BRE, BMP response element.