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HSAEC were split into membrane and cytosol fractions. iPLA2 activity was measured in the presence of R-enantiomer BEL (R-BEL) or S-BEL with or without tryptase (20 ng/ml, 10 min). Results are representative of means ± SE of 4 separate experiments. **P < 0.01 for S-BEL and tryptase or ++P < 0.01 for R-BEL and tryptase treated cells compared with cells treated with R- or S-BEL only.
