Fig. 2.

Mechanical strain inhibited lamellipodial extensions in migrating AT2 cells. Confluent monolayers were wounded, subjected to 0–15% CS (10 cpm) for 16 h, and fixed and stained with rhodamine-phalloidin for F-actin. Images were acquired using Z series, and lamellipodial extensions at the wound edge were traced and quantitated using Metamorph software. A: low-magnification images of cells at the wound edge as well as higher-magnification views of the indicated regions. Lamellipodial width (μm) was calculated as lamellipodial area/wound edge length as indicated (A, bottom). B: the measured lamellipodial width for static and 0–15% CS cells. Data are expressed as means ± SE of 3 independent experiments. #P < 0.05 vs. static control.