Fig. 1.

Animal breeding strategy and genotyping. A: the S23A mutation in p53 that is introduced by a single nucleotide substitution is represented. AD, transcriptional activation domain; DBD, DNA binding domain; OD, oligomerization domain. B: strategy of genetic crosses between p53^S23A × BdkrB2^−/− mice. BdkrB2, bradykinin B₂ receptor gene. C: PCR genotyping of S23A mice (right) was based on the presence of a single loxP site remaining in intron 1 of the correctly targeted locus. PCR genotyping of BdkrB2^−/− (left) shows the targeted allele (280 bp) and wild-type allele (361 bp).