Fig. 2.

Renal phenotype of newborn (P1) BdkrB2^−/− and BdkrB2^+/+ mice subjected to gestational high salt. Brown staining represents phospho-p53^S23 (p-p53^S23) distribution. A–C: salt-stressed BdkrB2^+/+ pups have normal overall kidney morphology, medullary development, and corticomedullar differentiation. P-p53^S23 is predominantly expressed in metanephric mesenchyme and nephron progenitors with lower levels in mature tubules. wt, Wild type. D–F: salt-stressed BdkrB2^−/− kidneys are cystic-dysplastic. P-p53^S23 is expressed diffusely and ectopically. G: Western blot analysis of p-p53^S23 and β-actin (loading control). Phospho-p53^S23 levels are approximately 3-fold higher in dysplastic than normal kidneys. Lane 1, p53^wt;BdkrB2^+/+; lane 2, p53^wt;BdkrB2^−/−.