Table 1.
Chemokines/cytokines in ischemic AKI kidneys
| Chemokine/cytokine, pg/mg | Sham Wild-Type (n = 5) | AKI Wild-Type (n = 8–10) | AKI IL-18BP Tg (n = 8–10) |
|---|---|---|---|
| IL-1α | 1.0±0.3 | 1.2±0.1 | 1.5±0.2 |
| IL-1β | 2±0.5 | 2.8±0.1 | 3.9±0.4† |
| IL-2 | 2.5±1.1 | 3.5±0.3 | 3.9±0.7 |
| IL-3 | 0.03±0.01 | 0.06±0.02 | 0.1±0.03* |
| IL-4 | 0.02±0.01 | 0.03±0.002 | 0.03±0.01 |
| IL-5 | 0.07±0.03 | 0.03±0.003 | 0.09±0.03 |
| IL-6 | 1.5±0.2 | 11.9±1.6* | 25.4±9.5* |
| IL-9 | 20.6±7.7 | 26.1±1.9 | 32±4.7 |
| 1L-10 | 1.6±0.2 | 1.7±0.1 | 2.2±0.4 |
| IL-12 (p40) | 0.9±0.1 | 1.1±0.1 | 1.3±0.1 |
| IL-13 | 7.3±1.6 | 7.2±0.7 | 9.1±1.7 |
| IL-15 | 3.8±1.8 | 20.2±2.2† | 20.3±2.2† |
| IL-17 | 0.4±0.06 | 0.4±0.04 | 0.5±0.09 |
| IL-18 | 86.4±42.1 | 129.4±15.9 | 192.3±23.2* |
| GM-CSF | 0.8±0.2 | 1.3±0.1 | 1.8±0.3† |
| G-CSF | 1.5±0.6 | 5.7±1.7 | 4.1±0.9 |
| M-CSF | 22.1±4.8 | 48±4.5* | 49.6±7.4* |
| IFN-γ | 1.2±0.2 | 1.3±0.1 | 1.8±0.3 |
| MIG | 22.5±10.5 | 26.9±2.7 | 35.6±3.9 |
| TNF-α | 4.3±1 | 4.3±0.3 | 5.9±0.9 |
| MIP-1α | 6.1±1 | 8.9±0.9 | 12.8±1.6† |
| MCP-1 | 4±0.3 | 15.6±2† | 15.3±2.5† |
| MIP-1α | 1.4±0.2 | 1.5±0.1 | 2.1±0.3 |
| MIP-2 | 0.5±0.4 | 40.6±6.5* | 31.7±6.1* |
| RANTES | 6.1±1.1 | 11.8±1 | 14.1±2 |
| Eotaxin | 14.2±5.2 | 18.5±1.5 | 23.7±3.0 |
| FGF-basic | 34.6±17.8 | 50.2±3.9 | 54.5±5 |
| LIF | 7.3±7.3 | 87.9±14.8* | 84.5±9.4* |
| CXCL1 (KC; IL-8) | 2.4±0.5 | 158.8±24.6‡ | 94.2±8.3§ |
| PDGF-BB | 24.3±12.4 | 52.6±6.3 | 55.6±9.2 |
| VEGF | 18.8±10.4 | 4.8±1.2 | 4±0.5* |
Values are means ± SE in sham-operated wild-type mouse kidneys (n = 5), acute kidney injury (AKI) wild-type mouse kidneys (n = 8–10), and AKI IL-18 binding protein transgenic (IL-18BP Tg) mouse kidneys (n = 8–10). GM-CSF, granulocyte-macrophage colony-stimulating factor; G-CSF, granulocyte colony-stimulating factor; M-CSF, macrophage colony-stimulating factor; IFN-γ, interferon-γ; MIG, monokine induced by IFN-γ; TNF-α, tumor necrosis factor-α; MIP-1, macrophage inflammatory protein-1; MCP-1, monocyte chemotactic protein-1; RANTES, regulated upon activation, normal T-cell expressed and secreted; FGF, fibroblast growth factor; LIF, leukemia inhibitory factor; CXCL1, chemokine (CXC) motif ligand 1 (also known as KC or IL-8); PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor.
P < 0.05;
P < 0.01;
P < 0.001 vs. sham.
P < 0.01 vs. AKI wild-type.