Fig. 9.

Effectiveness of KLF6 siRNA-mediated gene silencing. Thirty nanomolar KLF6 siRNA (Ambion) was transfected into HK-2 cells on 6-well plates with Lipofectamine 2000 according to manufacturer's instructions. In parallel, cells were transfected with 30 nM nonspecific siRNA, which served as the control (A). B and C: E-cadherin (B) and vimentin (C) expression in KLF6-overexpressing cells. Results are expressed as means ± SE. *P < 0.05, **P < 0.05 vs. control. All experimental conditions were in duplicate, and cell culture preparation was repeated 3 times, giving 6 individual measurements in each experimental group.