Figure 2.

Glycation abrogates the homologous restriction activity of human CD59. A 10% cell suspension of GPE was incubated with immunoaffinity-purified hRBC CD59 previously exposed for different time intervals to glucose (▵, 0.5 M) or ribose (□, 0.5 M), or to nonglycating sorbitol (■, 0.5 M). The sensitivity of GPE to human MAC was then tested by using purified human C5b6 and C7, C8, and C9. Each point represents the mean of triplicate determinations (SEM smaller than data points). Representative of three experiments with comparable results. (Inset) Number of CD59 molecules incorporated per GPE, determined with ¹²⁵I-CD59 before (C) and after (R) glycation with ribose.