Fig. 10.

β₁Flag and β₁myc subunits associate at detectable levels in MDCK/β₁flag/β₁myc cells. MDCK/β₁flag/β₁myc cells grown in media with (+) or without (−) Tet were harvested, and TM collected. TM (100 μg) was solublized in IPB containing 2% DDM. Two percent of the soluble protein were used for input, and the rest was immunoprecipitated with anti-flag antibody. Samples were treated with PNGase F, run on SDS-PAGE, and subject to Western blot analysis using anti-Myc antibody.