Fig. 5.

AA diameter responses to angiotensin (ANG) II (0.1, 1, 10 nM) in kidneys of db/m and db/db mice. Kidneys from db/m mice (db/m-NG; n = 12) were incubated in euglycemic (5 mM glucose) perfusion and superfusion solutions, while kidneys from db/db mice (db/db-HG; n = 12) were incubated in hyperglycemic (30 mM glucose) solutions. AA diameter responses to ANG II are plotted as the time course (A), average in micrometers (B), and Δ% of control (C). Afferent arterioles from db/m-NG and db/db-HG kidneys responded to increasing doses of ANG II with a significant reduction in diameter (P < 0.05). The magnitude of the AA vasoconstrictor responses was not significantly different between db/m-NG and db/db-HG mice. Data are means ± SE. *P < 0.05 vs. baseline diameter; †P < 0.05 db/m-NG vs. db/db-HG.