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PIP4Kγ is expressed in adult mouse tissues. Lysates were probed with PIP4Kγ-specific antibody (i) and with neutralized antibody after saturation with immunizing peptide (ii). Whole tissue lysates (A) and protein preparations from different kidney regions (B) were probed with the same antibody. Equivalent protein loading was based on total lysate concentration. Purified recombinant PIP4Kγ was used to identify endogenous PIP4Kγ at the correct molecular mass (arrow).
