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. 2008 May 21;295(5):F1271–F1285. doi: 10.1152/ajprenal.90252.2008

Fig. 1.

Fig. 1.

Computer-assisted reconstruction of loops of Henle from rat inner medulla (IM) showing expression of aquaporin-1 (AQP1; red) and ClC-K1 (green); gray regions (αB-crystallin) express undetectable levels of AQP1 and ClC-K1. Loops are oriented along the corticopapillary axis, with the left edge of each image nearer the base of the IM. A: thin limbs that have their bends within the first millimeter beyond the outer medullary (OM)-IM boundary. Descending segments lack detectable AQP1. ClC-K1 is expressed continuously along the prebend segment and the ATL. B: loops that have their bends beyond the first millimeter of the IM. AQP1 is expressed along the initial 40% of each descending thin limb (DTL) and is absent from the remainder of each loop. ClC-K1 is expressed continuously along the prebend segment and the ATL. Boxed area is enlarged in C. C: enlargement of near-bend regions of 4 thin limbs from box in B. ClC-K1 expression, corresponding to DTL prebend segment, begins, on average, 165 μm before the loop bend (arrows). Scale bars, 500 μm (A and B) and 100 μm (C). From Layton et al. (38).