Table 2.

The summary of single-channel kinetic analysis of the CT distributions from the wild-type receptor under control conditions and in the presence of fipronil

Agonist, modulator	CT1 (ms)	Fraction CT1	CT2 (ms)	Fraction CT2	CT3 (ms)	Fraction CT3	N
50 μM GABA	0.17±0.03	0.57±0.10	1.4±0.2	0.14±0.01	16.4±6.5	0.28±0.10	3
50 μM GABA+10 μM fipronil	0.21±0.05	0.54±0.13	1.6±0.5	0.16±0.05	11.9±1.9	0.30±0.10	4
1 mM GABA	0.24±0.02	0.69±0.04	0.9±0.1	0.29±0.04	7.8±4.0	0.02±0.01	4
1 mM GABA+10 μM fipronil	0.22±0.02	0.69±0.04	1.0±0.1	0.29±0.03	7.2±2.0	0.03±0.01	5

Abbreviation: CT, closed time.

The intracluster CT histograms were fitted to a sum of three exponentials. The table gives the mean durations (CT1–3) and relative contributions (fraction CT1–3) for the three CT components. The presence of fipronil did not lead to statistically significant changes in the CT distributions (P>0.05; Student's t-test). The CT2 component represents the activation-related CT (CTβ, see text) at 1 mM GABA. In the presence of 50 μM GABA, the CTβ coincides with the CT3 component.