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. 2008 Oct 21;99(10):1656–1667. doi: 10.1038/sj.bjc.6604712

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Copyright © 2008 Cancer Research UK

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Rac1 was implicated in the regulation of Du145 cell migration by Cyr61. (A) Protein levels of Cyr61 following forced expression of Cyr61 in Du145 cells. Lane a: Du145/WT cells; lane b: Du145 cells infected with lentivirus expressing GFP (Du145/GPF); lane c: Du145 cells infected with Cyr61 expression lentivirus (Du145/Cyr61). Activity of Rac1 was detected following silencing (down left) or forced expression of Cyr61 in Du145 cells (up right) and LnCap cells (down right) or treated with Rac1-specific inhibitor NSC23766. (B) Boyden chamber assay for either LnCap/GPF or LnCap/Cyr61 cells with or without priorly treated with NSC23766. ^**P<0.01. (C) Boyden chamber assay for either Du145/V or Du145/Cyr61 cells priorly treated with a series of concentrations of Rac1-specific inhibitor NSC23766. ^*P<0.05; ^**P<0.01. (D) Boyden chamber assay for either Du146/Ctrli or Du145/Cyr61i or Du145/Cyr61 transfected with Rac1 stimulator Tiam1. ^*P<0.01. (E) Rac1 downstream molecules that can affect cell migration and proliferation were detected both in Du145 cells and PC3 cells after silencing of Cyr61 expression. E-cadherin and p27^Kip1 protein level are elevated after silencing of Cyr61. However, protein levels of phosphorylated JNK (p-JNK) were decreased following silencing of Cyr61 expression in Du145 cells with two different siRNA sequences.