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. 2008 Oct 28;99(10):1668–1672. doi: 10.1038/sj.bjc.6604714

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Copyright © 2008 Cancer Research UK

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Detection of the fiber gene of AdV C genome by nested PCR. Serial 10-fold dilutions of the control DNA, ranging from 0.1 copy to 10³copies of the genome were used in the first reactions. Lane 1, molecular weight marker (100 bp); lanes 2–6, first round of amplification (0.1, 1, 10, 100 and 1000 copies, respectively); lanes 8–12, semi-nested amplification of the dilution series only; and lanes 7 and 13, negative controls.