Figure 4.

The proteasome inhibitor MG-132 sensitises AIPCa cells to the effects of IP6. In (A) (PC3) and (C) (DU145), cells were plated at 20 000 cells per well and pre-treated for 4 h with MG-132 (or ethanol control) before addition of IP6 at increasing concentrations. Metabolic activity was measured 24 h later using WST-1. For each cell line, relative metabolic activity was calculated according to the WST-1 output MG-132-treated or control cells treated with vehicle (0 mM IP6). Data represent an average of three independent experiments performed in three to six replicates each. ^***P-value<0.005; ^**P-value<0.01; ^#P-value=0.08; ^##P=0.09. (B) As described in Figure 1C, κB-luc reporter and control-transfected cells were treated with 2 or 20 μM MG-132 or vehicle (CTRL) and assayed for relative luciferase activity. Data represent an average of two independent experiments performed in four to eight replicates each. Error bars represent the standard error.