Figure 6.

Modulation of BCL-2 family protein expression in PC3 cells in response to IP6. MG-132 (MG) and cycloheximide (CHX). PC3 cells were pre-treated for 4 h with 20 μM MG-132, 50 μg ml⁻¹ cycloheximide or both. Cells were then challenged with 2 mM IP6 (or control) for 4 h after which whole-cell extracts were prepared and probed for MCL-1, BCL-2, IκB-α, PUMA, NOXA, BIK/NBK and BAX by western blot. GAPDH was used as a loading control. The presented blots were representative of three independent experiments each performed in duplicate.