Fig. 4.

Cx37-induced suppression of proliferation is density independent and has a rapid onset. iRin37-F cells were plated at 3 × 10⁴ cells per well. Doxycycline was added to one third of wells 24 h later (Dox+ data) and to another third 12 days later (Dox: 12d−, 9d+ data); the remaining wells were untreated (no Dox). Cell number was monitored at 3-day intervals for 21 days for all treatment groups. Proliferation of cells exposed to doxycycline was suppressed irrespective of cell density at the time of induction.