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Renal proximal tubules (RPTs) from adult (6 mo) and old (24 mo) Fischer 344 (F344) rats grow into epithelial cells in primary cultures. Epithelial cells grown on glass cover-slips (details in methods and materials) were scanned under microscope using ×20 phase (A) and ×60 fluorescence (B) objectives. Arrows in A show cell boundaries. Arrowheads in A and B show cell nuclei.
