Fig. 2.

Role of the COOH terminus in GR internalization and recycling: HEK-293T cells transfected with T7a (A) or T7b (B) containing plasmids were incubated in the absence (control) and presence of 100 nM glucagon for 30 min at 37°C. For recycling, the cells were washed and placed at 37°C for an additional 30 min. Surface GR was determined by radioligand binding assay (method B). The results shown are means ± SE of 3–4 independent experiments. The data were analyzed by one-way ANOVA and Bonferroni posttest. *,**,***Significantly different from respective control: P < 0.05, P < 0.01, and P < 0.001, respectively. ###Significantly different from 30-min glucagon: P < 0.001.