Fig. 5.

Effect of hypoxia on the abundance of p-AMPK, p-p38, and p-ERK1/2 in Clone 9 and H9C2 cells. A and B: Clone 9 cells. Confluent Clone 9 cells were exposed to hypoxia for 1 h, while cells treated with diluent, 10 mM azide, or 2 mM AICAR served as controls. C and D: H9C2 cells. Confluent H9C2 cells were exposed to hypoxia for 1 h, while cells treated with diluent, 10 mM azide, or 2 mM AICAR served as controls. The experiment was repeated 3 times; a representative blot is shown. Results of the blots were analyzed using densitometry and normalized against total AMPK, total p38, and total ERK1/2. Phosphorylation of AMPK, p38, and ERK1/2 is expressed as fold change compared with values in control cells. *P < 0.05 compared with individual control.