Fig. 5.

Transcriptional enhancement by −358/−173 AQP5 fragment in MLE-15 and NIH 3T3 cells. A: schematic diagram of DNA constructs showing insertion of single or multiple copies of −358/−173 AQP5 fragment in either forward or reverse orientations upstream of the AQP5 (−173-AQP5-Luc) or TK (TK-Luc) minimal promoters. B: transient transfections were performed in MLE-15 cells using the constructs shown in A. C: transient transfections were performed in NIH 3T3 cells using the constructs shown in A. Data represent means ± SE (n = 3). *P < 0.05 compared with −173-AQP5-Luc. Firefly luciferase was normalized to Renilla luciferase activity.