Skip to main content
. 2008 Sep 3;295(5):C1141–C1150. doi: 10.1152/ajpcell.00120.2008

Fig. 7.

Fig. 7.

GATA-6 activates −358-AQP5 promoter by interaction with Sp1. A: in transient transfection assays in S2 cells that lack Sp1, Sp1 activates −358-AQP5-Luc in a dose-dependent manner. Firefly luciferase was measured 24 h after transfection and normalized to β-galactosidase activity. Data represent means ± SE. *P < 0.05 compared with 0 ng of Sp1 (n = 3). B: NIH 3T3 cells were transfected with either Sp1-specific or control nontargeting small-interfering RNA (siRNA). Simultaneously, cells were transfected with −358-AQP5-Luc and pcDNA3/GATA-6 or empty vector pcDNA3. After 48 h, cells were harvested for Western analysis and luciferase assay. Firefly luciferase was normalized to total protein. Data represent means ± SE (n ≥ 3). *P < 0.05 compared with all other constructs. C: nuclear extract from MLE-15 cells was immunoprecipitated (IP) using anti-GATA-6 antibody or IgG. Sp1 associated with GATA-6 was detected by anti-Sp1 antibody as shown in this representative Western blot (WB). NE from MLE-15 cells is used as positive control for Sp1 Western blot.