Fig. 9.

Western blot of myosin heavy chain (MHC) isoforms. Anti-fast and anti-slow MHC primary antibodies were used to detect changes in extracted myosin myofilaments from wild-type (control) and mdm diaphragms at 2 and 6 wk (n = 3 for all age × genotype groups). At 2 wk, anti-fast MHC appeared similar in control and mdm diaphragms. Anti-slow MHC appeared to be slightly decreased in mdm diaphragms. At 6 wk, relative proportions of anti-fast MHC and anti-slow MHC detection were reversed in mdm diaphragm. Anti-slow MHC signal was much stronger in mdm diaphragm.