Figure 7.

Fas/Fas ligand gene expression in wild-type and TNFR1/TNFR2-deficient mice. Wild-type and TNFR1/TNFR2-deficient animals underwent acute coronary artery ligation or a sham operation. The animals were killed 3 h after coronary artery ligation, mRNA was isolated from the frozen hearts, and ribonuclease protection assays were performed by using custom designed probes for murine FADD-like ICE (FLICE), Fas ligand, FADD, and Fas-associated factor (FAF). A representative ribonuclease protection assay for wild-type (WT) and TNFR1/TNFR2-deficient mice (KO) is shown in A. (B) Summary of the results of group data, expressed as the ratio of the OD of the Fas/Fas ligand-related genes normalized by the OD for L32, a housekeeping gene.