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ANG II but not ANG-(1-7) regulates ACE2 mRNA. VSMCs in defined serum-free media were incubated for 12 h with increasing concentrations of ANG II or ANG-(1-7) (A) or for increasing times in the presence of 100 nM ANG II or ANG-(1-7) (B). EDTA (0.5 mmol/l) was included to prevent the degradation of ANG II by ACE2, ACE, or neprilysin. ACE2 mRNA was measured by reverse transcriptase, real-time PCR. n = 4.
