Fig. 1.

Gel analysis of reverse-transcription PCR products of oxytocin (OT) and vasopressin (VP) primary transcripts and transcription intermediates from the rat supraoptic nucleus. Reverse-transcription PCR products were loaded on to a 1.5% agarose gel containing ethidium bromide for gel electrophoresis. The arrows in the schematic diagrams in A–C show the binding sites of the specific primers for reverse transcription. The arrowheads in the panels show the binding sites of the PCR primer pairs to the predicted cDNAs derived from the OT and VP transcripts in the rat SON. A: RT-PCR products derived from the OT and VP primary transcripts. B: RT PCR products derived from transcription intermediates containing only intron 1 of the OT or VP gene. C: RT-PCR products derived from the transcription intermediates containing only intron 2 of the OT or VP gene. The strongest band in the 100 bp ladder marker is 500 bp. The primers used for the reverse transcription and the PCR, as well as the predicted amplicon lengths are shown in Supplemental Table S1. All the products have the predicted sizes of the RNA forms.