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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Immunity. 2008 Oct 30;29(5):691–703. doi: 10.1016/j.immuni.2008.08.016

Figure 7.

Figure 7

Hes1 negatively regulates IL-6 expression

(A) Fetal liver-derived macrophages from wild-type, Hes1-haplodeficient or Hes1-deficient embryos were stimulated with 10 ng/ml of Pam3Cys. Cytokine mRNA expression was determined by real time PCR. Data are shown as means + SD of triplicate determinants and are representative of two independent experiments.

(B) IL-6 protein concentrations in supernatants of Pam3Cys-stimulated fetal liver-derived macrophages were analyzed by ELISA. Results are representative of two independent experiments.

(C) RAW264.7 cells were cotransfected in duplicate with the full length IL-6 reporter construct and a Hes1 expression plasmid or empty vector control. 24 h after transfection, cells were stimulated with 1 μg/ml of LPS for 6 h, and cell lysates were analyzed for luciferase activity. Data are shown as mean + SD from duplicate transfections and are representative of three independent experiments.