Immunogold labeling of GABA and glutamate receptors in spine and shaft synapses innervated by GABAergic terminals in TTX-treated cerebella. (A) GABAergic terminal showing two synapses: one with the shaft, devoid of labeling, and another one with a spine expressing GluR1. (B) GABAA α1 subunits are expressed in both shaft (arrowheads) and spine (arrow) synapses. s, spine; PD, proximal dendrite. (Scale bar, 0.5 μm.)