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. 2008 Dec 5;4(12):e1000289. doi: 10.1371/journal.pgen.1000289

Figure 6. Progressive epithelial dysfunction in Kindlin-1−/− mice.

Figure 6

(A) Normal morphology of IECs and BM at E18.5. Shown are H&E stainings of the ileum and colon and electron microscopy pictures at 12000× magnification from the colon. The boxed enlargement shows the BM, e: epithelium. Arrows point to the BM. (B) Colonic IEC (e) detachment at P0 (Colon P0, around 5–6 hours after birth) that becomes infiltrated by immune cells around 12 hours after birth (Colon P0.5). In mice delivered by Caesarean section (CS) and kept unfed for 7 hours no epithelial cell detachment can be observed. Arrows indicate blister. (C) IEC detachment but no macrophage (Mac-1) and granulocyte (GR-1) infiltrations at P0 (Mac-1 and GR-1 in green; Perlecan (Perl) indicating BM in red). Arrows indicate IEC detachment. (D) Immune cell infiltrations in the lumen of the colon and floating epithelial sheets (e) in the colonic lumen at P1. PAS staining shows reduced goblet cell mucins in Kindlin-1−/− colonic epithelium. Scale bars in A, B and D represent 50 µm and in C 10 µm. (E) Median of Real Time PCR results from whole colon mRNA at E18.5 (n = 2 per genotype) and P1 (n = 5 per genotype) for TNF-α and IL-6. Error bars show range. The P value was determined using a Mann-Whitney test.