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. Author manuscript; available in PMC: 2008 Nov 19.
Published in final edited form as: Science. 2008 Oct 3;322(5898):110–115. doi: 10.1126/science.1158111

Fig. 2.

Fig. 2

Role of ceramide in CEP-1/p53–mediated germ cell apoptosis. (A) hyl-1 and lagr-1 are epistatic to abl-1 in germ cell apoptosis. Germ cell apoptosis was scored in abl-1(ok171), hyl-1(ok976);abl-1(ok171), lagr-1(gk327);abl-1(ok171), and lagr-1(gk327);hyl-1(ok976);abl-1(ok171) at 36 hours (left) and in lagr-1(gk327);hyl-1(ok976);abl-1(ok171) at the indicated times after exposure to 120 Gy (right). Data (mean ± SEM, represented by error bars) are collated from 10 to 15 worms per group. (B) hyl-1 and lagr-1 deletions do not affect 120-Gy–induced p53-mediated egl-1 (left) and ced-13 (right) up-regulation measured by reverse transcription polymerase chain reaction. Data (mean ± SEM) are compiled from three experiments. (C) Baseline EGL-1 is required for C16-ceramide (∼1 μM gonadal ceramide)–induced germ cell apoptosis. Studies were performed as in Fig. 1B. Data (mean ± SEM) are collated from ≥15 worms per group.