Figure 5. Hh maintains Odd expression near the En cells.

(A, A′) Stage 11 hh^AC embryo stained for En (green), Odd (red) and Slp (blue). Odd is still present whereas En disappears.

(B, B′) Stage 12 hh^AC embryo stained for En (green), Odd (red) and cadherin (blue). Some En expressing cells remain, but no Odd cells are in the epidermis. Some Odd staining is visible in the nervous system.

(C, C′) Stage 12 ptc^IN embryo stained for En (green), Odd (red) and cadherin (blue). The Odd domain is wider than in the wild-type (compare with Figure 4H)

(D, D′) Stage 13 En-gal4 UASHh^WT embryo stained for En (green), Odd (red) and cadherin (blue). Odd is present in a four to five cell wide stripe.

(E) Detail of a stage 11 wild-type embryo stained for Ptc (green), Cadherin (red) and Odd (blue). Both Ptc (E′) and Odd (E″) domains are several cells wide.

(F) Detail of a stage 13 wild-type embryo stained for Ptc (green), Cadherin (red) and Odd (blue). Both the Ptc (E′) and the Odd (E″) domains are a single cell wide.

(G, G′, G″) Stage 13 ptc^IN embryo stained for En (green), aPKC (red) and cadherin (blue). Crb is enriched in the widened grooves. En face (G′) and Z-section (G″) detail from (G).

Note the groove on the left has ectopic En posteriorly, while the groove on the right does not. For 3D rotation movie, see Movie 4 in Supplemental Material.