Figure 2.
Detection of AIS expression by Northern analysis. (A) AIS transcripts in HNSCC cell lines and lung cancer cell lines. AIS expression is observed in all HNSCC cell lines, whereas no expression is observed in the SaOs2 cell line. A human β-actin probe was used as an internal control. (B) Samples (10 μg) of total RNA extracted from tumor (T) and normal (N) tissues of five different patients with primary lung cancers were hybridized with a 32P-labeled probe for AIS or β-actin. AIS expression of various intensities is observed in the tumor RNA of patients L2, L10, and L12 but is absent from all normal tissue controls.