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Small-scale agarose gel electrophoresis using MCF-7 and MDA-MB-231 nuclear extracts. 32P-labeled ERE-containing oligos were incubated with nuclear extracts from MCF-7 breast cancer cells, which endogenously express ERα (A), or nuclear extracts from MDA-MB-231 breast cancer cells and purified ERα (B). An ERα-specific antibody was included in the binding reaction as indicated. Complexes were resolved on agarose gels and visualized by autoradiography.
