Fig. 4.

Reconstitution of DKO mice by adoptive T cell transfer and homing to tumors. a T cell reconstitution in DKO mice receiving no cells, naïve (unchallenged) T cells, or primed T cells (from WT animals that were initially challenged with tumor) was determined as a proportion of all viable cells using spleen samples as in Fig. 2. Data show the means ± SD (measured in four WT mice and three mice from each DKO group) for CD3 (gray), CD4 (black), and CD8 (white) T cells in reconstituted spleens. Similar results were obtained when the analysis was performed in lymph nodes. b Representative dot plots for CD4 and CD8 staining in spleens of control B6, B6 DKO, or B6 DKO mice transferred with naïve T cells, and B6 DKO mice transferred with T cells primed by exposure to tumor in the absence of FasL (AE-NR). Tumors were available for staining from the same DKO and DKO+ naïve mice, and are shown in the bottom middle and right panels. Percentages for CD4 and CD8 cells, respectively, in each panel were: 35/17 (WT B6 spleen), 0.4/0 (DKO spleen + no adoptive transfer), 27/1 (DKO spleen + naïve T cells), 30/21 (DKO spleen + primed AE-NR T cells), 0/0 (DKO tumor + no adoptive transfer), 7/0.3 (DKO tumor + naïve T cells)