Table 1.
Time to islet rejectiona
| Recipient | Donor strain | |||
|---|---|---|---|---|
| NOD | C57Bl/6 | |||
| Median (N) | Mean (±SD) | Median | Mean (±SD) | |
| WT | >60 (7) | >60 | >60 (4) | >60 |
| SCID | 45 (4) | 45b (17) | >60 (4) | >60 |
| β2m (class I knockout) | >60 (5) | 54c (21.0) | >60 (2) | >60 |
| C2D (class II knockout) | >60 (2) | >60 | >60 (3) | >60 |
| DKO (class I + II knockout) | 18d (4) | 18.5d (9.0) | 9.5d (4) | 9.75d (3.3) |
aRecipient mice received 2 × 107 donor T cells by intravenous injection. After 4 weeks, endogenous pancreatic islets were ablated with streptozotocin, and diabetes was corrected by transplantation of wild-type islets under the kidney capsule. Blood glucose concentrations were used to monitor pancreatic islet function; values >6 mM were indicative of islet graft rejection. Experiments were terminated 60 days after islet transplantation, considered a hallmark of long-term graft survival
bPancreatic grafts in two of four mice functioned >60 days
cTwo animals rejected islet grafts in <60 days, one at 19 days and one at 52 days
dSignificantly different from control (P < 0.05)