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. 2008 Oct 16;27(22):2943–2954. doi: 10.1038/emboj.2008.211

Figure 3.

Figure 3

Capping protein inhibits VASP-mediated actin assembly in solution. (A, D) TIRF images of actin filaments polymerized for 10 min with either DdVASP or hVASP (200 nM each) in the presence and absence of Cap32/34 or CapZ (40 nM each) in 1 × TIRF buffer, respectively. White arrows in the TIRF images (left) and corresponding kymographs (right) indicate filaments that stopped growing in the presence of CP. The growth of non-capped filaments is shown for comparison. Scale bar, 10 μm. (B, E) Cap32/34 and CapZ decrease the average length of actin filaments formed in the presence of DdVASP or hVASP. Filaments formed in the presence of VASP were longer than the actin control. (C, F) The time to growth arrest is virtually independent of VASP. The times of filament elongation until capping were calculated from the elongation rates of VASP-mediated actin assembly. The lengths of at least 150 filaments for each condition were measured. Note different scales in B and E. Data correspond to means±s.e.m.