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Western blotting and dual-color SIFT measurement of prion rod dilution. (A) Brain homogenate of scrapie-infected hamster 263K (lanes a–f) and prion rod material (lanes g–o) in CSF was diluted as indicated. PrPSc was detected by Western blotting with 3F4 antibody after digestion with proteinase K (100 μg/ml) for 30 min at 37°C. (B) In parallel, aliquots of prion rod material were measured by dual-color SIFT, and the peak signal was quantified as in Fig. 2.
