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Dual-color SIFT measurements in CSF samples. Measurements were performed for a measurement time of 600 s with a channel time of 500 μs with mAbs 3F4-Alexa488 and 12F10-Cy5 and analyzed as described in Fig. 3. By using a cutoff level of one high-intensity channel, a specific dual-color, high-intensity signal was found in 5 of 24 patients with probable or definite CJD, whereas none of the CSF samples from 13 non-CJD cases with neurodegenerative disease contained a positive signal.
