Inactivation of prelimbic cortex, BLA, or NAcore did not reinstate cocaine seeking after 11 d of extinction training, but inactivation of prelimbic cortex and BLA prevented reinstatement induced by inactivating the infralimbic cortex. The left shows the active and inactive lever pressing on each day of the experiment, and the right shows the average extinction baseline pressing and pressing after microinjecting PBS control or baclofen plus muscimol (B+M) into each structure shown in A–C. A, A two-way ANOVA of prelimbic data showed only a significant effect of lever (F(1,8) = 5.14, p = 0.053). B, A two-way ANOVA of BLA data showed no significant effects. C, A two-way ANOVA of NAcore data showed significant effects of treatment (F(1,14) = 6.67, p = 0.021) and lever (F(1,14) = 4.69, p = 0.048) but no interaction. D, The left shows the average extinction baseline pressing and pressing after microinjecting PBS control or baclofen plus muscimol (B+M) into the prelimbic cortex, and the right into BLA, in conjunction with a microinjection of B+M into infralimbic cortex. A two-way ANOVA of the dual inactivation of the infralimbic cortex and prelimbic cortex show significant effects of treatment (F(1,26) = 6.86, p = 0.015), lever (F(1,16) = 10.63, p = 0.003), and a significant interaction (F(1,26) = 4.34, p = 0.047). A two-way ANOVA of the dual inactivation of the infralimbic cortex and BLA show significant effects of treatment (F(1,16) = 13.67, p = 0.002), lever (F(1,16) = 17.86, p < 0.001), and a significant interaction (F(1,16) = 15.01, p = 0.001). Numbers in bars indicate n. *p < 0.05 comparing PBS and B+M (A–C) or inactivation of both brain regions versus infralimbic inactivation alone (D).