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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Neurochem. 2008 Sep 15;107(4):1001–1013. doi: 10.1111/j.1471-4159.2008.05685.x

Fig. 1.

Fig. 1

Acute ethanol-induced formation of [3H]-AEA and 2-AG. (A–D) Neurons in culture (15 DIV) were labeled with [3H]-AA (1 μCi/ml) in neurobasal media for 22 hours and then treated with or without ethanol (50 mM) for up to 60 minutes. Cells were treated with or without SR141716A(SR) (2μM) or EGTA (10 mM) in the presence or absence of ethanol (50 mM) for 30 minutes. [3H]-AEA and 2-[3H]-AG were extracted from the medium and cells (total), and separated by TLC using solvent system A (n = 8). *P< 0.05, **P< 0.001, ***P< 0.0001.