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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Neurochem. 2008 Sep 15;107(4):1001–1013. doi: 10.1111/j.1471-4159.2008.05685.x

Fig. 3.

Fig. 3

ECs mediate ethanol-induced suppression of mini excitatory postsynaptic currents. (A) Hippocampal neurons in culture (20x). Whole-cell patch-clamp recordings were made from a single pyramidal-shaped neuron. (B) Traces of continuously recorded mEPSCs before (control), during ethanol exposure, and after the addition of the CB1 receptor antagonist SR141716A (SR). (C) In the presence of 50 mM ethanol for 20 min, the frequency was reduced (inter-event intervals) without affecting the amplitude (p<0.0001) (n= 12 neurons). [↓, Vehicle, ethanol or SR was added to the bath solution; ↓↓, SR was added to the bath solution]. (D) Combined plots of the average amplitudes of mEPSCs under control, ethanol, ethanol + SR141716A, and SR141716A treatment conditions (n= 12 neurons) (E) Combined plots of the average inter-event-intervals of mEPSCs under control, ethanol, ethanol + SR141716A, and SR141716A treatment conditions (n= 12 neurons) (***p<0.0001). Average cumulative distributions of mEPSC (F) inter-event intervals (sec) (p < 0.01) in ethanol-treated cells relative to controls (Kolmogorow-Simrnov two-sample test).