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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Hepatol. 2008 May 22;49(2):251–261. doi: 10.1016/j.jhep.2008.04.015

Figure 3. The extracellular matrix modulates hepatocyte size.

Figure 3

A. The average cell area of hepatocytes plated for 4 hr on Type 1 (I) or Type IV (IV) collagen or fibronectin (FB), laminin (LM) or polylysine (PL) was determined. Cells were fixed and stained, and areas of at least 50 randomly selected cells were measured from phase contrast photomicrographs as described in the methods. Results represent mean and standard deviation of at least 3 separate experiments. The * indicates the values are significantly different from hepatocytes plated on I (p<0.05). 3B. The amount of apoptosis induced by 2 hr treatment of hepatocytes with 50 uM GCDC was compared to average hepatocyte cell area on different ECM. Results represent mean and standard deviation of at least 3 separate experiments. Apoptosis was quantified as % of 500 randomly counted Hoechst stained cell cells